The primary cell isolate was prepared from human tissue obtained with full ethical permission. Cells were isolated by enzymatic digestion and cultured in optimal conditions for keratinocyte growth. Cells were banked and cryopreserved under liquid nitrogen. The cell population was analysed by fluorescence-activated flow cytometry.
Donor Tissue Features
Female donor, age 32 years
Isolated from normal abdominal skin
Additional donor history available on request
Batch number: 15-2503
Vial content: 0.5x106 cells
Appearance: Sheet of flat cells with central nuclei
Seeding density: 5,000-6,000 cells/cm2
Culture medium: GibcoTM SF Keratinocyte medium recommended
Recovery from frozen: 97% on Type I collagen
Population doubling: 7-8 days
Negative for Mycoplasma, HIV1, HIV2, Hepatitis B and Hepatitis C
Negative for yeast, bacteria and fungus
Uses and restrictions
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