Human Primary Dermal Fibroblasts; DF-HN-184 #14-2509 Datasheet

 

This primary cell isolate was prepared from human tissue obtained with full ethical permission. Skin tissue was dissected to obtain explants of dermis which were seeded into tissue culture treated dishes. Cell outgrowth from explanted tissue was promoted using customised medium optimized for primary human dermal fibroblast cells. A fibroblast population was isolated using FSP-1 immuno-magnetic selection. Propagated cells were banked and cryopreserved under liquid nitrogen. The cell population was analysed by fluorescence-activated flow cytometry.


 

Donor Tissue Features

Female donor, age 62 years

Isolated from normal abdominal skin

Additional donor history available on request

 

Cell characteristics

Batch number:  14-2509

Vial content:  0.5x106 cells

Appearance:  Monolayer of spindle-shaped cells

Seeding density:  5,000-7,000 cells/cm2

Culture medium:  AvantiCell medium DF-HNM-01 recommended

Recovery from frozen:  96%

Population doubling:  2-3 days

Negative for Mycoplasma, HIV1, HIV2, Hepatitis B, Hepatitis C 

Negative for yeast, fungus and bacteria

 

                           

Interstitial fibroblast marker CD73 99.9%
Myofibroblast marker aSMA 50.8%

 

 

Uses and restrictions

  • Store at -150°C. Once thawed do not re-freeze
  • For research use ONLY — not suitable for in vitro diagnostic use or human or animal treatment
  • Potential biohazard — handle with care

 

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