Human Primary Cervical Cancer Cells; CV-HC-030 #17-0706 Datasheet

 

This human primary cervical cancer cell isolate was prepared from cervical cancer tissue obtained with full ethical permission. Cervical cancer tissue was dissociated by enzymatic digestion and cells were harvested and washed by conventional filtration and centrifugation. Cells were grown in culture using medium optimized for propagation of primary cervical epithelial cells, and were banked and cryopreserved under -150oC. Cell population analysis was performed by fluorescence-activated flow cytometry.


 

Donor Tissue Features

Female Malaysian donor, age 54 years

Isolated from cervical cancer stage 3B

Additional donor history available on request

 

Cell Characteristics

Batch number:  17-0706

Vial content:  0.5x106 cells

Appearance:  Simple cervical epithelial cells

Seeding density:  5,000 - 7,000 cells/cm2

Culture medium:  ScienCell CerEpiCM recommended

Recovery from frozen:  94%

Population doubling:  3-4 days

Negative for Mycoplasma,  HIV1, HIV2, Hepatitis B and Hepatitis C

Negative for yeast, fungus and bacteria

 

                              

Cancer stem cell marker CD133 0.33%
Cervical cancer cell marker p16INK4a 91.22%
Metastasis marker  CD44 96.06%

 

 

Uses and restrictions

  • Store at -150°C. Once thawed do not re-freeze
  • For research use ONLY — not suitable for in vitro diagnostic use or human or animal treatment
  • Potential biohazard — handle with care

 

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