Human Primary Pulmonary Artery Fibroblasts; AF-HN-138 #12-1611 Datasheet

 

This primary cell isolate was prepared from human tissue obtained with full ethical permission. Human pulmonary artery was treated enzymatically and the collected cells were characterized by flow cytometric analysis of cell markers. Fibroblasts were further selected using FSP-1 labelled immuno-magnetic beads and the harvested fibroblast cell population was grown in medium optimized for primary human pulmonary arterial fibroblasts. Cells were banked by cryopreservation under liquid nitrogen.


 

Donor Tissue Features

Male donor, age 38 years

Isolated from pulmonary artery

Positive for CMV and EBV

Additional donor history available on request

 

Cell Characteristics

Batch number:  12-1611

Vial content:  0.5x106 cells

Appearance:  Monolayer of fibroblast shaped cells

Seeding density:  6,000 cells/cm2

Culture medium:  AvantiCell medium AF-HNM-01 recommended

Recovery from frozen:  70%       

Doubling time:  5 days

Negative for Mycoplasma, HIV1, HIV2, Hepatitis B, Hepatitis C and STS

Negative for yeast, fungus and bacteria

 

                                     

Intestinal fibroblast marker CD73 97.13%
Myofibroblast marker aSMA 80.48%

 

 

Uses and restrictions

  • Store at -150°C. Once thawed do not re-freeze
  • For research use ONLY — not suitable for in vitro diagnostic use or human or animal treatment
  • Potential biohazard — handle with care

 

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