Human Primary Tracheal Epithelial Cells; LE-HN-081 #12-1611T Datasheet

 

The primary cell isolate was prepared from human tissue obtained with full ethical permission. Cells were isolated by enzymatic digestion and cultured in optimal conditions for epithelial growth. Cells were banked and cryopreserved under liquid nitrogen. The cell population was analysed by fluorescence-activated flow cytometry.


 

Donor Tissue Features

Male Caucasian donor, age 38 years

Isolated from airway trachea

Positive for EBV IgG and CMV 

Additional donor history available on request

 

Cell Characteristics

Batch number:  12-1611T

Vial content:  0.5x106 cells

Appearance:  Flat cells with central nuclei

Seeding density:  5,000 - 6,000 cells/cm2

Surface coating:  Human type IV collagen

Culture medium:  BEGMTM (Lonza)

Recovery from frozen:  91%

Doubling time:  2 days

Negative for Mycoplasma, HIV1, HIV2, Hepatitis B, Hepatitis C and STS

Negative for yeast, fungus, bacteria

 

                                        

Epithelial cell marker EpCAM 96.0%
Epithelial cell marker E-Cadherin 76.0%

 

 

Uses and restrictions

  • Store at -150°C. Once thawed do not re-freeze
  • For research use ONLY — not suitable for in vitro diagnostic use or human or animal treatment
  • Potential biohazard — handle with care

 

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