This primary cell isolate was prepared from human tissue obtained with full ethical permission. Cells were isolated by enzymatic digestion and cultured in optimal conditions for epithelial growth. Cells were banked and cryopreserved under liquid nitrogen. The cell population was analysed by fluorescence-activated flow cytometry.
Donor Tissue Features
Male Caucasian donor, age 38 years
Isolated from airway primary bronchi
Positive for EBV IgG and CMV
Additional donor history available on request
Cell Characteristics
Batch number: 12-1611
Vial content: 0.5x106 cells
Appearance: Rounded flat cells with central nuclei
Seeding density: 5,000 - 6,000 cells/cm2
Culture medium: BEGMTM (Lonza)
Recovery from frozen: 85%
Doubling time: 3-4 days
Negative for Mycoplasma, HIV1, HIV2, Hepatitis B, Hepatitis C and STS
Negative for yeast, fungus and bacteria
Epithelial cell marker | EpCam | 86.4% |
Epithelial cell marker | E-Cadherin | 49.1% |
Uses and restrictions
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