This primary cell isolate was prepared from human tissue obtained with full ethical permission. Cells were isolated by enzymatic digestion and cultured in optimal conditions for epithelial growth. Cells were banked and cryopreserved under liquid nitrogen. The cell population was analysed by fluorescence-activated flow cytometry.

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Donor Tissue Features

Male Caucasian donor, age 38 years

Isolated from airway primary bronchi

Positive for EBV IgG and CMV

Negative for HIV1, HIV2, Hepatitis B, Hepatitis C and STS

Additional donor history available on request

 

Cell Characteristics

Batch number:  12-1611

Vial content:  0.5x10⁶ cells

Appearance:  Rounded flat cells with central nuclei

Seeding density:  5,000 - 6,000 cells/cm²

Culture medium: Lonza BEGM^TM Bronchial Epithelial Cell Growth Medium

Substratum:  Human type IV collagen

Recovery from frozen:  85%

Doubling time:  3-4 days

Negative for Mycoplasma

Negative for yeast, fungus and bacteria

 

                                                    

Epithelial cell marker	EpCam	86.4%
Epithelial cell marker	E-Cadherin	49.1%

 

 

Uses and restrictions

• Store at -150°C. Once thawed do not re-freeze
• For research use ONLY — not suitable for in vitro diagnostic use or human or animal treatment
• Potential biohazard — handle with care

 

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