Human Primary Lung Cancer Fibroblasts; LF-HC-088 #17-0112 Datasheet

 

This primary cell isolate was prepared from tissue obtained with full ethical permission. Lung tumour tissue was dissected and dissociated by proteolytic digestion. A fibroblast population was isolated using FSP-1 immuno-magnetic selection and propagated in customised fibroblast culture medium. Cells were banked and cryopreserved under -150oC. Cell population analysis was performed by fluorescence-activated cell sorting (FACS).


 

Donor Tissue Features

Female donor, age 83 years

Isolated from stage pT2 N0 M0 lung adenocarcinoma

Additional donor history available on request

 

Cell Characteristics

Batch number:  17-0112

Vial content:  0.5x106

Appearance:  Monolayer of fibroblast shaped cells

Seeding density:  12,000/cm2

Culture medium:  AvantiCell medium LF-HCM-01 recommended

Recovery from frozen:  96%

Doubling time:  2 days

Negative for Mycoplasma, Hepatitis B, Hepatitis C, HIV1 and HIV2 

Negative for fungus, bacteria and yeast

 

                                  

Fibroblast surface marker FSP 94%
Myofibroblast marker aSMA 96%

 

 

Uses and restrictions

  • Store at -150°C. Once thawed do not re-freeze
  • For research use ONLY — not suitable for in vitro diagnostic use or human or animal treatment
  • Potential biohazard — handle with care

 

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