Human Primary IPF Lung Fibroblasts; LF-HD-087 #15-1305 Datasheet

 

This primary cell isolate was prepared from tissue obtained with full ethical permission. Tissue was dissected and dissociated by proteolytic digestion. A fibroblast population was isolated using FSP-1 immunomagnetic selection and propagated in customised fibroblast culture medium. Cells were banked, cryopreserved and stored at -150oC. Cell population analysis was performed by fluorescence-activated flow cytometry.

Read our Technology Overview for example data using AvantiCell IPF lung fibroblasts.


 

Donor Tissue Features

Female donor, age 64 years

Isolated from IPF airway bronchial tree

Additional donor history available on request

 

Cell Characteristics                                                    

Batch number:  15-1305

Vial content:  0.5x106

Appearance:  Elongated spindle shaped cells

Seeding density:  3,000 - 4,000 cells/cm2

Population doubling:  2-3 days

Culture medium:  AvantiCell medium LF-HDM-01 recommended

Recovery from frozen:  99%

Negative for Mycoplasma, HIV1, HIV2, Hepatitis B and Hepatitis C

Negative for fungus, yeast and bacteria

 

                                 

Interstitial fibroblast marker CD73 99.48%
Myofibroblast marker aSMA 51.01%

 

 

Uses and restrictions

  • Store at -150°C. Once thawed do not re-freeze
  • For research use ONLY — not suitable for in vitro diagnostic use or human or animal treatment
  • Potential biohazard — handle with care

 

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