This primary cell isolate was prepared from tissue obtained with full ethical permission. Tissue was dissected and dissociated by proteolytic digestion. A fibroblast population was isolated using FSP-1 immunomagnetic selection and propagated in customised fibroblast culture medium. Cells were banked, cryopreserved and stored at -150oC. Cell population analysis was performed by fluorescence-activated flow cytometry.
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Donor Tissue Features
Female donor, age 64 years
Isolated from IPF airway bronchial tree
Additional donor history available on request
Batch number: 15-1305
Vial content: 0.5x106
Appearance: Elongated spindle shaped cells
Seeding density: 3,000 - 4,000 cells/cm2
Population doubling: 2-3 days
Culture medium: AvantiCell medium LF-HDM-01 recommended
Recovery from frozen: 99%
Negative for Mycoplasma, HIV1, HIV2, Hepatitis B and Hepatitis C
Negative for fungus, yeast and bacteria
|Interstitial fibroblast marker||CD73||99.48%|
Uses and restrictions
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