Human Primary IPF Lung Fibroblasts; LF-HD-087 #15-1305 Datasheet

 

This primary cell isolate was prepared from tissue obtained with full ethical permission. Tissue was dissected and dissociated by proteolytic digestion. A fibroblast population was isolated using FSP-1 immunomagnetic selection and propagated in customised fibroblast culture medium. Cells were banked, cryopreserved and stored at -150oC. Cell population analysis was performed by fluorescence-activated flow cytometry.


 

Donor Tissue Features

Female donor, age 64 years

Isolated from IPF airway bronchial tree

Additional donor history available on request

 

Cell Characteristics                                                    

Batch number:  15-1305

Vial content:  0.5x106

Appearance:  Elongated spindle shaped cells

Seeding density:  3,000 - 4,000 cells/cm2

Population doubling:  2-3 days

Culture medium:  AvantiCell medium LF-HDM-01 recommended

Recovery from frozen:  99%

Negative for Mycoplasma, HIV1, HIV2, Hepatitis B and Hepatitis C

Negative for fungus, yeast and bacteria

 

                                 

Interstitial fibroblast marker CD73 99.48%
Myofibroblast marker aSMA 51.01%

 

 

Uses and restrictions

  • Store at -150°C. Once thawed do not re-freeze
  • For research use ONLY — not suitable for in vitro diagnostic use or human or animal treatment
  • Potential biohazard — handle with care

 

To download this information, click here.

You may also need

Idiopathic Pulmonary Fibrosis Lung Fibroblast Medium; LF-HDM-01 Datasheet

More Info

Human Primary IPF Lung Fibroblasts; LF-HD-087 #15-0705 Datasheet

More Info