Human Primary IPF Lung Fibroblasts; LF-HD-087 #15-0705 Datasheet

 

This primary cell isolate was prepared from human tissue obtained with full ethical permission. Tissue was dissected and dissociated by proteolytic digestion. A fibroblast population was isolated using FSP-1 immuno-magnetic selection and propagated in customised fibroblast culture medium. Cells were banked by cryopreservation under liquid nitrogen. The cell population was analysed by fluorescence-activated flow cytometry.

Read our Technology Overview for example data using AvantiCell IPF lung fibroblasts.


 

Donor Tissue Features

Male donor, age 61 years

Isolated from IPF airway bronchial tree

Additional donor history available on request

 

Cell Characteristics

Batch number:  15-0705

Vial content:  0.5x106 cells

Appearance:  Elongated spindle shaped cells

Seeding density:  3,000 – 4,000 cells/cm2

Population doubling:  2 days

Culture medium:  AvantiCell medium LF-HDM-01 recommended

Recovery from frozen:  >90% viability

Negative for Mycoplasma, HIV1, HIV2, Hepatitis B and Hepatitis C 

Negative for fungus, yeast and bacteria

 

                                      

Interstitial fibroblast marker CD73 99.46%
Myofibroblast marker aSMA 83.45%

 

 

Uses and restrictions

  • Store at -150°C. Once thawed do not re-freeze
  • For research use ONLY — not suitable for in vitro diagnostic use or human or animal treatment
  • Potential biohazard — handle with care

 

To download this information, click here.

You may also need

Idiopathic Pulmonary Fibrosis Lung Fibroblast Medium; LF-HDM-01 Datasheet

More Info

Human Primary IPF Lung Epithelial Cells; LE-HD-085 #15-0705 Datasheet

More Info

Human Primary IPF Lung Fibroblasts; LF-HD-087 #15-1305 Datasheet

More Info