Human Primary IPF Lung Epithelial Cells; LE-HD-085 #15-0705 Datasheet

 

This primary cell isolate was prepared from human tissue obtained with full ethical permission. Cells were isolated by enzymatic digestion and cultured in optimal conditions for epithelial growth. Fibroblasts were removed from culture using negative FSP-1 immunomagnetic selection Cells were banked and cryopreserved under liquid nitrogen. The cell population was analysed by fluorescence-activated flow cytometry.


 

Donor Tissue Features

Male donor, age 61 years

Isolated from IPF airway bronchial tree

Additional donor history available on request

 

Cell Characteristics

Batch number:  15-0705

Vial content:  0.5x106 cells

Appearance:  Rounded flat cells with central nuclei

Seeding density:  5,000-6,000 cells/cm2

Population doubling:  2-3 days

Culture medium:  BEGMTM (Lonza)

Recovery from frozen:   93% viability

Negative for Mycoplasma, HIV1, HIV2, Hepatitis B and Hepatitis C

Negative for fungus, yeast and bacteria

 

                            

Epithelial cell marker EpCam 81.2%
Epithelial cell marker E-Cadherin 27.4%

 

 

USES AND RESTRICTIONS

  • Store at -150°C. Once thawed do not re-freeze
  • For research use ONLY — not suitable for in vitro diagnostic use or human or animal treatment
  • Potential biohazard — handle with care

 

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