Human Primary Cystic Fibrosis Lung Epithelial Cells; LE-HD-083 #17-2403 Datasheet

 

This primary cell isolate was prepared from human tissue obtained with full ethical permission. Cells were isolated by enzymatic digestion and cultured in optimal conditions for epithelial growth. Cells were banked and cryopreserved under liquid nitrogen. The cell population was analysed by fluorescence-activated cell sorting (FACS) and immunocytochemistry.


 

Donor Tissue Features

Male donor, age 39 years

Isolated from cystic fibrosis airway bronchi 

Additional donor history available

 

Cell Characteristics

Batch number:  17-2403

Vial content:  0.5x106 cells

Appearance:  Flat cells with central nuclei

Seeding density:  5000-6000 cells/cm2

Culture medium:  BEGMTM (Lonza)

Recovery from frozen:  89%

Population doubling:  1-2 days

Negative for Mycoplasma, HIV1, HIV2, Hepatitis B, Hepatitis C, Hepatitis A, HTLV1 and HTLV2 

Negative for yeast, bacteria and fungus

Positive for CFTR 

 

                                   

Epithelial cell marker EpCam 73.1%
Epithelial cell marker E-Cadherin 59.2%

 

 

Uses and restrictions

  • Store at -150°C. Once thawed do not re-freeze
  • For research use ONLY — not suitable for in vitro diagnostic use or human or animal treatment
  • Potential biohazard — handle with care

 

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