Human Primary COPD Lung Epithelial Cells; LE-HN-082 #11-0608 Datasheet

 

This primary cell isolate was prepared from human tissue obtained with full ethical permission. Cells were isolated by enzymatic digestion and cultured in optimal conditions for epithelial growth. Cells were banked and cryopreserved under liquid nitrogen. The cell population was analysed by fluorescence-activated flow cytometry.


 

Donor Tissue Features

Female donor, age 44 years

Isolated from COPD airway bronchi

Additional donor history available on request

 

Cell Characteristics

Batch number:  11-0608

Vial content:  0.5x106 cells

Appearance:  Flat cells with central nuclei

Seeding density:  5,000 - 6,000 cells/cm2

Culture medium:  BEGMTM recommended (Lonza)

Recovery from frozen:  86%

Doubling time:  2-3 days

Negative for Mycoplasma, Hepatitis B, Hepatitis C, HIV1 and HIV2

Negative for fungus, bacteria and yeast

 

                                         

Epithelial cell marker EpCam 54.0%
Epithelial cell marker E-Cadherin 80.4%

 

 

Uses and restrictions

  • Store at -150°C. Once thawed do not re-freeze
  • For research use ONLY — not suitable for in vitro diagnostic use or human or animal treatment
  • Potential biohazard — handle with care

 

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