Human Primary Renal Medulla Fibroblasts ; KF-HN-051 #16-2106 Datasheet


This primary cell isolate was prepared from human tissue obtained with full ethical permission. Medulla tissue was mechanically dissociated into small explants which were cultured under conditions optimised for primary kidney fibroblast cells. A fibroblast population was isolated using FSP-1 immuno-magnetic selection. The selected population was expanded, banked, and cryopreserved under liquid nitrogen. The cell population was analysed by fluorescence-activated flow cytometry.

Read our Technology Overview for example data using AvantiCell renal fibroblasts.


Donor Tissue Features

Male Caucasian donor, age 53 years

Isolated from healthy kidney medulla, no evidence of abnormal pathology

Negative for Hepatitis B, Hepatitis C, HIV1, HIV2 and CMV

Positive for EBV IgG

Additional donor history available on request


Cell Characteristics

Batch number:  16-2106

Vial content:  0.5x106 cells

Appearance:  Sheet of elongated cells

Seeding density:  4,000-5,000 cells/cm2

Culture medium:  AvantiCell medium KF-HNM-01 recommended

Recovery from frozen:  94%

Negative for Mycoplasma

Negative for yeast, bacteria and fungus



Fibroblast surface marker FSP 58.1%
Myofibroblast marker aSMA 54.7%



Uses and restrictions

  • Store at -150°C. Once thawed do not re-freeze
  • For research use ONLY — not suitable for in vitro diagnostic use or human or animal treatment
  • Potential biohazard — handle with care


To download this information, click here.

You may also need

Renal Fibroblast Medium; KF-HNM-01 Datasheet

More Info

Human Primary Renal Fibroblasts; KF-HN-051 #16-2106 Datasheet

More Info