This primary cell isolate was prepared from human tissue obtained with full ethical permission. Medulla tissue was mechanically dissociated into small explants which were cultured under conditions optimised for primary kidney fibroblast cells. A fibroblast population was isolated using FSP-1 immuno-magnetic selection. The selected population was expanded, banked, and cryopreserved under liquid nitrogen. The cell population was analysed by fluorescence-activated flow cytometry.
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Donor Tissue Features
Male Caucasian donor, age 53 years
Isolated from healthy kidney medulla, no evidence of abnormal pathology
Additional donor history available on request
Batch number: 16-2106
Vial content: 0.5x106 cells
Appearance: Sheet of elongated cells
Seeding density: 4,000-5,000 cells/cm2
Culture medium: AvantiCell medium KF-HNM-01 recommended
Recovery from frozen: 94%
Negative for Mycoplasma, Hepatitis B, Hepatitis C, HIV1, HIV2 and CMV
Positive for EBV IgG
Negative for yeast, bacteria and fungus
|Fibroblast surface marker||FSP||58.1%|
Uses and restrictions
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