Human Primary Renal Medulla Fibroblasts ; KF-HN-051 #16-2106 Datasheet

 

This primary cell isolate was prepared from human tissue obtained with full ethical permission. Medulla tissue was mechanically dissociated into small explants which were cultured under conditions optimised for primary kidney fibroblast cells. A fibroblast population was isolated using FSP-1 immuno-magnetic selection. The selected population was expanded, banked, and cryopreserved under liquid nitrogen. The cell population was analysed by fluorescence-activated flow cytometry.


 

Donor Tissue Features

Male Caucasian donor, age 53 years

Isolated from healthy kidney medulla, no evidence of abnormal pathology

Additional donor history available on request

 

Cell Characteristics

Batch number:  16-2106

Vial content:  0.5x106 cells

Appearance:  Sheet of elongated cells

Seeding density:  4,000-5,000 cells/cm2

Culture medium:  AvantiCell medium KF-HNM-01 recommended

Recovery from frozen:  94%

Negative for Mycoplasma, Hepatitis B, Hepatitis C, HIV1, HIV2 and CMV

Positive for EBV IgG 

Negative for yeast, bacteria and fungus

 

                                              

Fibroblast surface marker FSP 58.1%
Myofibroblast marker aSMA 54.7%

 

 

Uses and restrictions

  • Store at -150°C. Once thawed do not re-freeze
  • For research use ONLY — not suitable for in vitro diagnostic use or human or animal treatment
  • Potential biohazard — handle with care

 

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