Human Primary Renal Fibroblasts; KF-HN-051 #16-2106 Datasheet

 

The primary cell isolate was prepared from human tissue obtained with full ethical permission. Cortex tissue was mechanically dissociated into small explants which were cultured under conditions optimised for primary kidney fibroblast cells. A fibroblast population was isolated using FSP-1 immuno-magnetic selection. The selected population was expanded, banked, and cryopreserved under liquid nitrogen. The cell population was analysed by fluorescence-activated flow cytometry.

Read our Technology Overview for example data using AvantiCell renal fibroblasts.


 

Donor Tissue Features

Male Caucasian donor, age 53 years

Isolated from healthy kidney cortex, no evidence of abnormal pathology

Additional donor history available on request

 

Cell Characteristics

Batch number:  16-2106

Vial content:  0.5x106 cells

Appearance:  Sheet of elongated cells

Seeding density:  4,000-5,000 cells/cm2

Culture medium:  AvantiCell medium (KF-HNM-01) recommended

Recovery from frozen:  96%

Population doubling:  1-2 days

Negative for Mycoplasma, Hepatitis B, Hepatitis C, HIV1, HIV2, CMV

Positive for EBV IgG 

Negative for yeast, bacteria, fungus

 

                                           

Fibroblast surface marker FSP 54.2%
Myofibroblast marker aSMA 73.9%

 

 

Uses and restrictions

  • Store at -150°C. Once thawed do not re-freeze
  • For research use ONLY — not suitable for in vitro diagnostic use or human or animal treatment
  • Potential biohazard — handle with care

 

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