The primary cell isolate was prepared from human tissue obtained with full ethical permission. Cortex tissue was mechanically dissociated into small explants which were cultured under conditions optimised for primary kidney fibroblast cells. A fibroblast population was isolated using FSP-1 immuno-magnetic selection. The selected population was expanded, banked, and cryopreserved under liquid nitrogen. The cell population was analysed by fluorescence-activated flow cytometry.
Read our Technology Overview for example data using AvantiCell renal fibroblasts.
Donor Tissue Features
Male Caucasian donor, age 53 years
Isolated from healthy kidney cortex, no evidence of abnormal pathology
Additional donor history available on request
Batch number: 16-2106
Vial content: 0.5x106 cells
Appearance: Sheet of elongated cells
Seeding density: 4,000-5,000 cells/cm2
Culture medium: AvantiCell medium (KF-HNM-01) recommended
Recovery from frozen: 96%
Population doubling: 1-2 days
Negative for Mycoplasma, Hepatitis B, Hepatitis C, HIV1, HIV2, CMV
Positive for EBV IgG
Negative for yeast, bacteria, fungus
|Fibroblast surface marker||FSP||54.2%|
Uses and restrictions
To download this information, click here.