This primary cell isolate was prepared from human tissue obtained with full ethical permission. Tissue was mechanically dissociated into small explants which were cultured under conditions optimized for primary kidney fibroblast cells. Fibroblasts were recovered in cells out-growing from the explants. A fibroblast population was isolated using FSP-1 immuno-magnetic selection. The population was expanded, banked, and cryopreserved under liquid nitrogen. The cell population was analysed by fluorescence-activated flow cytometry.
Read our Technology Overview for example data using AvantiCell renal fibroblasts.
Donor Tissue Features
Female Caucasian donor, age 63 years
Isolated from adjacent normal kidney cortex
Additional donor history available on request
Batch number: 12-1109BF
Vial content: 0.5x106 cells
Appearance: Sheet of elongated cells
Seeding density: 4,000-5,000 cells/cm2
Culture medium: AvantiCell medium (KF-HNM-01) recommended
Recovery from frozen: 97%
Population doubling: 4-5 days
Negative for Mycoplasma, HIV1, HIV2, Hepatitis B and Hepatitis C
Negative for yeast, bacteria and fungus
|Fibroblast surface marker||FSP||90.6%|
Uses and restrictions
To download this information, click here.