Human Primary Kidney Fibroblasts; KF-HN-051 #12-1109BF Datasheet

 

This primary cell isolate was prepared from human tissue obtained with full ethical permission. Tissue was mechanically dissociated into small explants which were cultured under conditions optimized for primary kidney fibroblast cells. Fibroblasts were recovered in cells out-growing from the explants. A fibroblast population was isolated using FSP-1 immuno-magnetic selection. The population was expanded, banked, and cryopreserved under liquid nitrogen. The cell population was analysed by fluorescence-activated flow cytometry.

Read our Technology Overview for example data using AvantiCell renal fibroblasts.


 

Donor Tissue Features

Female Caucasian donor, age 63 years

Isolated from adjacent normal kidney cortex

Additional donor history available on request

 

Cell Characteristics

Batch number:  12-1109BF

Vial content:  0.5x106 cells

Appearance:  Sheet of elongated cells

Seeding density:  4,000-5,000 cells/cm2

Culture medium:  AvantiCell medium (KF-HNM-01) recommended

Recovery from frozen:  97%

Population doubling:  4-5 days

Negative for Mycoplasma, HIV1, HIV2, Hepatitis B and Hepatitis C

Negative for yeast, bacteria and fungus

 

                                          

Fibroblast surface marker FSP 90.6%
Myofibroblast marker aSMA 91.5%

 

 

Uses and restrictions

  • Store at -150°C. Once thawed do not re-freeze
  • For research use ONLY — not suitable for in vitro diagnostic use or human or animal treatment
  • Potential biohazard — handle with care

 

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