The primary cell isolate was prepared from human tissue obtained with full ethical permission. Tissue was mechanically dissociated into small explants which were cultured under conditions optimized for primary kidney fibroblast cells. Fibroblasts were recovered in cells out-growing from the explants. A fibroblast population was isolated using FSP-1 immuno-magnetic selection. The population was expanded, banked, and cryopreserved under liquid nitrogen. The cell population was analysed by fluorescence-activated flow cytometry.
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Donor Tissue Features
Female Black African donor, age 60 years
Isolated from adjacent normal kidney cortex
Additional donor history available on request
Batch number: 12-1109F
Vial content: 0.5x106 cells
Appearance: Sheet of elongated cells
Seeding density: 4,000-5,000 cells/cm2
Culture medium: AvantiCell medium KF-HNM-01 recommended
Recovery from frozen: 95%
Population doubling: 2-3 days
Negative for Mycoplasma, HIV1, HIV2, Hepatitis A, Hepatitis B, Hepatitis C, HTLV1 and HTLV2
Negative for fungus, bacteria and yeast
|Fibroblast surface marker||FSP||71%|
Uses and restrictions
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