The primary cell isolate was prepared from human tissue obtained with full ethical permission. Tissue was mechanically dissociated into small explants which were cultured under conditions optimized for primary kidney fibroblast cells. Fibroblasts were recovered in cells out-growing from the explants. A fibroblast population was isolated using FSP-1 immuno-magnetic selection. The population was expanded, banked, and cryopreserved under liquid nitrogen. The cell population was analysed by fluorescence-activated flow cytometry.

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Donor Tissue Features

Female Black African donor, age 60 years

Isolated from adjacent normal kidney cortex

Additional donor history available on request

 

Cell Characteristics

Batch number:  12-1109F

Vial content:  0.5x10⁶ cells

Appearance:  Sheet of elongated cells

Seeding density:  4,000-5,000 cells/cm²

Culture medium:  AvantiCell medium KF-HNM-01 recommended

Recovery from frozen:  95%

Population doubling:  2-3 days

Negative for Mycoplasma, HIV1, HIV2, Hepatitis A, Hepatitis B, Hepatitis C, HTLV1 and HTLV2

Negative for fungus, bacteria and yeast

 

                                         

Fibroblast surface marker	FSP	71%
Myofibroblast marker	aSMA	97%

 

 

Uses and restrictions

• Store at -150°C. Once thawed do not re-freeze
• For research use ONLY — not suitable for in vitro diagnostic use or human or animal treatment
• Potential biohazard — handle with care

 

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