This primary cell isolate was prepared from human tissue obtained with full ethical permission. Tissue was dissected and treated with collagenase and chelation. The resulting cells were propagated in colon epithelia media. Cells were banked and cryopreserved under liquid nitrogen. The cell population was analysed by fluorescence-activated flow cytometry.

---

 

Donor Tissue Features

Male Caucasian donor, age 64 years

Isolated from Crohn’s Disease diagnosed small bowel

Additional donor history available on request

 

Cell Characteristics

Batch number:  11-1125C

Vial content:  0.5x10⁶ cells

Appearance:  Large irregular cells

Seeding density:  9,000 cells/cm²

Culture medium:  AvantiCell medium CE-HDM-01 recommended

Recovery from frozen:  95%

Population doubling:  2 days

Negative for Mycoplasma, HIV1, HIV2, Hepatitis B and Hepatitis C

 

                                            

Epithelial cell marker	E-Cadherin	3.0%
Intestinal epithelia-associated marker	CD73	98.9%

 

 

Uses and restrictions

• Store at -150°C. Once thawed do not re-freeze
• For research use ONLY — not suitable for in vitro diagnostic use or human or animal treatment
• Potential biohazard — handle with care

 

To download this information, click here.