Human Primary Colon Cancer Cells; CE-HC-105 #12-0427C Datasheet

 

This primary cell isolate was prepared from human tissue obtained with full ethical permission. Tissue was dissected and treated with collagenase and chelation. Cells were propagated in colon cancer culture medium. Cells were banked and cryopreserved under liquid nitrogen. The mixed cell population was analysed by fluorescence-activated flow cytometry.


 

Donor Tissue Features

Male Caucasian donor, age 64 years

Isolated from colon tumour

Additional donor history available on request

 

Cell Characteristics

Batch number:  12-0427C

Vial content:  0.5x106 cells

Appearance:  Large irregular cells

Seeding density:  6,000 cells/cm2

Culture medium:  AvantiCell medium CE-HCM-01 recommended

Recovery from frozen:  80%

Population doubling:  1-2 days

Negative for Mycoplasma, HIV1, HIV2, Hepatitis B and Hepatitis C 

Negative for yeast, bacteria, fungus

 

                                            

Stem cell marker CD133 0.09%
Tumour cell marker CD73 99.2%

 

 

Uses and restrictions

  • Store at -150°C. Once thawed do not re-freeze
  • For research use ONLY — not suitable for in vitro diagnostic use or human or animal treatment
  • Potential biohazard — handle with care

 

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