Human Primary Colon Cancer-Associated Fibroblasts; CF-HC-106 #12-0427C Datasheet

 

The primary cell isolate was prepared from human tissue obtained with full ethical permission. Tissue was dissected and treated with collagenase and chelation. A colon cancer associated fibroblast population (CAF) was isolated using FSP-1 selection and the resulting cells were propagated in customized colon fibroblast medium. Cells were banked and cryopreserved under liquid nitrogen. The cell population was analysed by fluorescence-activated flow cytometry.


 

Donor Tissue Features

Male donor, age 64 years

Isolated from colon tumour

Additional donor history available on request

 

Cell Characteristics

Batch number:  12-0427C

Vial content:  0.5x106 cells

Appearance:  Large irregular cells

Seeding density:  2,000-3,000 cells/cm2

Culture medium:  AvantiCell medium CF-HCM-01 recommended

Recovery from frozen:  81%

Population doubling:  2-3 days

Negative for Mycoplasma, HIV1, HIV2, Hepatitis B and Hepatitis C

Negative for yeast, bacteria, fungus

 

                                             

Myofibroblast CAF-associated marker aSMA 84%
Fibroblast associated marker FSP-1 21%

 

 

Uses and Restrictions

  • Store at -150°C. Once thawed do not re-freeze
  • For research use ONLY — not suitable for in vitro diagnostic use or human or animal treatment
  • Potential biohazard — handle with care

 

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