Human Primary Breast Cancer Cells; BE-HC-061 #11-1905 Datasheet

 

This primary cell isolate was prepared from human tissue obtained with full ethical permission. Tissue was dissociated by enzymatic digestion and cells were harvested and washed by conventional filtration and centrifugation. Cells were grown in culture using medium optimised for primary breast cancer cells, and were banked and cryopreserved under liquid nitrogen.  The cell population was analysed by fluorescence-activated flow cytometry.


 

Donor Tissue Features

Female donor, age 49 years

Isolated from tumour stage AJCC pT1, lymph node status positive (0/11). In situ lobular carcinoma with comedo structures

Additional donor history available

Cell Characteristics

Batch number:  11-1905

Vial content:  0.5x106 cells

Seeding density:  13,333 cells/cm2

Culture medium:  AvantiCell medium BE-HCM-01 recommended

Recovery from frozen:  >90%

Population doubling:  2-3 days   

Negative for Mycoplasma, HIV1, HIV2, Hepatitis A, Hepatitis B, Hepatitis C, HTLV1 and HTLV2

Negative for yeast, bacteria and fungus

 

                                              

Oestrogen receptor Luminal Positive
Stem cell-related marker CD24/CD44 Positive
Epithelial cell marker EpCam Positive
Disease prognosis indicator HER2 Positive

 

 

Uses and restrictions

  • Store at -150°C. Once thawed do not re-freeze
  • For research use ONLY — not suitable for in vitro diagnostic use or human or animal treatment
  • Potential biohazard — handle with care

 

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