Human Primary Glioblastoma Cancer Cells; GM-HC-074 #11-1309 Datasheet

 

This primary cell isolate was prepared from human tissue obtained with full ethical permission. Tissue was dissociated by enzymatic digestion and cells were harvested and washed by conventional filtration and centrifugation. Cells were grown in culture using medium optimised for primary glioblastoma cancer cells, and were banked and cryopreserved under liquid nitrogen.  Cell population analysis was performed by fluorescence flow cytometry.


 

Donor Tissue Features

Male donor, age 75 years

Isolated from glioblastoma multiforme tumour stage IV

Additional donor history available on request

 

Cell Characteristics

Batch number:  11-1309

Vial content:  0.5 x 106 cells

Appearance:  Dependent upon culture conditions

Seeding density:  1.35 a x105 cells/cm2 (adherent culture), 2 x 105 cells/ml (suspension culture)

Culture medium:  AvantiCell medium GM-HCM-01 recommended

Recovery from frozen:  >90%

Negative for Mycoplasma, HIV1, HIV2, Hepatitis A, Hepatitis B, Hepatitis C, HTLV1 and HTLV2 

Negative for fungus, bacteria and yeast

 

                                                

Cancer Stem Cell Marker CD33 Negative
Neuronal progenitor cell marker CD56 75%
Glial progenitor cell marker A2B5 25%

 

 

Uses and restrictions

  • Store at -150°C. Once thawed do not re-freeze
  • For research use ONLY — not suitable for in vitro diagnostic use or human or animal treatment
  • Potential biohazard — handle with care

 

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