Human Primary CD8+ Cytotoxic T-Cells; CD8-HN-201 #18-1012 Datasheet


The CD8+ Cytotoxic T-cells were isolated from a human peripheral blood mononuclear cells (PBMCs) fraction of human whole blood, obtained with full ethical permission. The cell population was analysed by flow cytometry.  The CD8+ Cytotoxic T-cells were immediately frozen post-isolation and cryopreserved under liquid nitrogen.


Donor Tissue Features

Male Caucasian donor, age 65 years

Isolated from whole blood fraction


Cell Characteristics

Batch Number:  18-1012

Vial Content:  2.5 x 106 cells

Appearance:  Suspension of round cells

Seeding Density: 8 x 105 cells/ml

Culture Medium:  RPMI + 10% human serum 

Recovery:  90%

Negative for Mycoplasma, HIV-1, HIV-2, Hepatitis B and Hepatitis C by real-time PCR

Negative for fungus, yeast, bacteria


Antigen Cell Surface Glycoprotein CD8 96%



Uses and restrictions

  • Store at -150°C. Once thawed do not re-freeze
  • For research use ONLY — not suitable for in vitro diagnostic use or human or animal treatment
  • Potential biohazard — handle with care

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