The CD14+ monocytes were isolated from a fraction of human whole blood, obtained with full ethical permission. The cell population was isolated via density-gradient separation of peripheral blood mononuclear cells followed by immuno-magnetic selection of CD14+ cell population. Cell marker analysis confirmed the CD14+ status of the cells and the monocytes were cryopreserved and stored under vapour phase liquid nitrogen.
Donor Tissue Features
NHS-accredited, Leukoreduction System Chamber (LRSC)
Isolated from the whole blood fraction
Catalogue Code: MN-HN-301
Batch Number: 19-0609B
Vial Content: 5 x 106 cells
Appearance: Suspension of round cells
Seeding Density: 1 x 106 cells/ml
Culture Medium: AvantiCell MN-HNM-01 recommended
Tested negative for Mycoplasma, HIV1, HIV2, Hepatitis B and Hepatitis C
Negative for fungus, yeast and bacteria
For more information on how CD14+ monocytes were isolated, read our Technology Overview.
Uses and restrictions